1. Biosecurity Responsibility

Name of Biosecurity Coordinator: John Doe. Credentials. 

The Biosecurity Coordinator is responsible for reviewing the Biosecurity Plan at least once per year. All changes made to the Biosecurity Plan will be documented and dated on the last page of the Biosecurity Plan, titled Biosecurity Plan Changes. At times of heighten risk (need to define heightened risk, examples: when avian influenza is present in neighboring states and/or when farm is in the control zone of an avian influenza outbreak) the Biosecurity Coordinator will review the Biosecurity Plan and track any changes made. 

2. Training

All employees will be trained on farm’s biosecurity plans at the time of hire. New employees sign off on the biosecurity trainings and signed copies of the training will be filed in each employee’s file. In addition to the training at hire, all employees will have continuing training at least once a year, which they will also sign off on. (Include what type of training materials are used. Training materials may include DVD’s, PowerPoints, and handouts and can be found in the biosecurity folder. http://poultrybiosecurity.org/)

It must be stated in your plan how long you retain training records. The NPIP requirement is a minimum of 3 years. 

3. Line of Separation (LOS) 

The LOS can be found labeled on the map following the Biosecurity Plan. Generally, the LOS is the walls of the chicken house/flight pen nets.  

• Employees entering the LOS: 
  • Practice good hygiene, including showering daily and wearing freshly laundered clothes.
  • May not have any contact with non-company owned birds.
  • Leave any personal items outside the LOS
  • Wear farm designated footwear
  • Step into footbath upon entry and exit of the LOS
• Visitors entering the LOS:
  • Must park in the designated parking area with all car windows closed
  • Must first check in with a farm employee
  • Cannot have contact with poultry 72 hours before coming to the farm, unless other arrangements have been made
  • Must wear farm designated PPE, including coveralls and boot covers
  • Must wash/sanitize hands
  • Must step into footbath upon entry and exit of the LOS 

4. Perimeter Buffer Area (PBA) – What kind of cleaner do you use? 

The PBA is labeled on the map with the LOS. Generally, the PBA is a ten foot surrounding area of any LOS, as well as any buildings that are used daily for poultry production. The PBA is to be kept mowed and debris free. 

• Any equipment crossing the PBA must be cleaned and disinfected with ____ cleaner prior to crossing into the PBA. 
• Any contract workers should check in with farm personnel prior to entering the PBA. Contract workers should wear farm designated clothing and sign into the visitor log. 

5. Personnel

Site dedicated personnel should follow the above LOS crossing procedures. Employees must wear freshly laundered, clean clothes, site specific footwear, and wash/sanitize hands. 

Visitors will be provided with personal protective equipment (PPE) (this is just an example, could provide just disposable boots, list whatever you do. PPE includes Tyvek coveralls, plastic booties, and hairnets.) Hands should be washed/sanitized before entering the LOS. Any drivers or other contract employees should wear site specific footwear and sanitize hands. Third party contract employees sign off that they will follow farm biosecurity practices. 

Anyone that has come in contact with poultry within 48-72 hours will not be permitted access to farm birds.  Also, anyone that is feeling ill should not have contact with farm birds. 

Employees, visitors, and contract workers should limit activities that could contaminate clothing or vehicles with AI virus including: 

• Working at or visiting other poultry sites
• Hunting or contact with wild gamebirds or wild waterfowl
• Living with people who work at other premises with poultry
• Visiting homes with other backyard poultry or waterfowl
• Walking through areas contaminated with waterfowl or wild gamebird feces

6. Wild Birds, Rodents, & Insects

Wild birds, rodents, & insects are monitored by a visual inspection. Any holes/cracks in building siding or foundation are to be repaired as soon as possible. Baits/traps are placed around the farm and checked daily by visual inspection (put more details here such as type of traps/bait stations and type of bait used). If rodent or insect levels are too high (define what acceptable/not acceptable level is for you), corrective actions (can list what those may be, ex: change bait type, add more bait stations), will be taken and documented. 

7. Equipment and Vehicles 

Vehicles that are used inside the PBA are cleaned and disinfected on a weekly basis or on an as needed basis. If equipment is shared between farms, the inside and outside is cleaned and disinfected prior to crossing the PBA.

Cleaning SOP:

• Remove all visible debris
• Wash with water and detergent
• Thoroughly rinse the cleaned area to remove detergent residue
• Allow item to dry completely
• Apply an appropriate disinfectant
• Allow proper contact time. Contact time may vary based on what disinfectant is used but is usually around 10 minutes

Vehicle patterns are attached on the map of the farms. The driveway is graveled and signs stating bio secure area are posted before reaching the PBA. 

8. Mortality Disposal—How are they disposed?, Where do you store the dead before being disposed? 

Mortality is collected from the barns at least once per day. Mortality should be stored away from the live birds until it is able to be disposed of either by composting, incineration, trash pickup, or rendering- Pick what you do. The storage and disposal method should limit the accessibility of rodents and insects to the mortality. Any storage of dead birds is kept in a secure closed container. If the level of rodents and insects grows too high, bait/trap stations will be used. 

9. Manure and Litter Management—What is done with the manure? Do you do anything with the barns before placing new birds? Where do you store the manure? 

Any manure storage is done so in a separate closed area to prevent attraction of rodents and insects. Once the manure is removed from the barn, any equipment used for the removal is cleaned and disinfected. Records are kept for any manure sold off the farm. Companies and crews bringing new litter onto the farm have a COC stating they will follow farm biosecurity. 

10. Replacement Poultry

Replacement poultry is sourced from company owned flocks. Any replacement poultry bought originates from an NPIP flock with a VS Form 9-3. All VS Form 9-3s are kept in the office for at least three years. Any equipment used for placing replacement poultry is cleaned and disinfected prior and after use. Any third party crews have a COC stating that they follow farm biosecurity. 

11. Water Supply

Water is sourced through ________. Water is tested once a year for pH and mineral content. 

12. Feed and Replacement Litter— How is feed stored? 

Feed is sourced from a third party company. Any third party trucks and crews for feed have a COC stating that they follow farm biosecurity. Any feed spills within the PBA are to be cleaned up immediately as to not attract any wild birds. 

13. Reporting of Elevated Morbidity/Mortality—What is the point when you would call OPA and take AI samples? 

Elevated mortality for farm is defined as __Need an actual number or percentage_______. Once this level is met or if there is any suspicious mortality or morbidity, the farm manager will call the Ohio Poultry Association until they reach one of the following: 

Site map can be hand drawn or an image of the farm and should include:

• Parking areas
• Traffic routes & driveways— if multiple driveways, where do visitors enter? Where do feed trucks enter? 
• LOS
• PBA
• Mortality storage area(s)
• Manure and Litter storage 
• Feed Bins
• Label buildings
• Anything else that may be involved in daily activities of farm

Standard E – Biosecurity Principles

Based on the flock size as stated in the 9 CFR 53.10, the following minimum management practices and principles are designed to prevent the introduction and spread of infectious diseases.

(1) Biosecurity responsibility

The Biosecurity Coordinator is responsible for the development, implementation, maintenance and ongoing effectiveness of the biosecurity program. Depending on the type and size of poultry operation, the Biosecurity Coordinator’s responsibility could be at the farm, production site, production complex, or company level. The Biosecurity Coordinator should be knowledgeable in the principles of biosecurity. The Biosecurity Coordinator, along with the personnel and caretakers on the farms and production sites are responsible for the implementation of the biosecurity program. The Biosecurity Coordinator should review the biosecurity program at least once during each calendar year and make revisions as necessary.

(2) Training

The biosecurity program should include training materials that cover both farm site-specific procedures as well as premises-wide and/or company-wide procedures as appropriate. All poultry owners and caretakers that regularly enter the perimeter buffer area (PBA) must complete this training. The training must be done at least once per calendar year and documented. New poultry caretakers should be trained at hire. Training records should be retained as stated in Title 9-CFR §145.12(b) and 146.11(e).

(3) Line of Separation (LOS)

The Line of Separation (LOS) is a functional line separating the poultry house(s) and the poultry inside from exposure to potential disease sources. Generally, it is defined by the walls of the poultry building with practical deviations to account for entry points, structural aspects, or outside access areas. The site-specific biosecurity plan should describe or illustrate the boundaries of the LOS and clearly outline the procedures to be followed when caretakers, visitors, or suppliers cross it.

For poultry enclosed in outdoor pens, similar principles for the LOS can be applied for defining and controlling the LOS for each pen. In this circumstance, the walls of the outdoor pens would provide template for defining the LOS to be used when entering or exiting the pens.

For poultry with non-enclosed outdoor access, the LOS is recommended but not required. Further, in an emergency disease state where the transmissible disease risk is heightened, it is highly recommended to enclose all poultry and enforce a LOS.

(4) Perimeter Buffer Area (PBA)

The perimeter buffer area is a functional zone surrounding the poultry houses or poultry raising area that separates them from areas unrelated to poultry production on that site and/or adjoining properties. It is comprised of the poultry houses and poultry raising areas as well as nearby

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structures and high traffic areas involved in the daily function of the poultry farm. This would usually include but not be limited to such things as feed bins, manure sheds, composting areas, egg rooms, generators, pump rooms, etc. The site-specific biosecurity plan should describe or illustrate the boundaries of the PBA and clearly outline the procedures that caretakers, visitors, or suppliers must follow when entering and leaving the PBA.

(5) Personnel

The biosecurity program and/or the site-specific biosecurity plan should include provisions specifically addressing procedures and biosecurity PPE for site-dedicated personnel. The plan should likewise address the procedures and biosecurity PPE for non-farm personnel. The plan should also specify procedures which all personnel having had recent contact with other poultry or avian species should follow before re-entering the PBA.

(6) Wild Birds, Rodents and Insects

Poultry operations should have control measures to prevent contact with and protect poultry from wild birds, their feces and their feathers as appropriate to the production system. These procedures should be reviewed further during periods of heightened risks of disease transmission. Control programs for rodents, insects, and other animals should be in place and documented.

(7) Equipment and Vehicles.
The biosecurity plan should include provisions for procedures for cleaning, disinfection, or restriction of sharing of equipment where applicable. Vehicle access and traffic patterns should be defined in the site-specific biosecurity plan.

(8) Mortality Disposal

Mortality should be collected daily, stored and disposed in a manner that does not attract wild birds, rodents, insects, and other animals and minimizes the potential for cross-contamination from other facilities or between premises. It is recommended that dead bird disposal be on-site, if possible. Mortality disposal should be described in the site-specific biosecurity plan.

(9) Manure and Litter Management

Manure and spent litter should be removed, stored and disposed of in a manner to prevent exposure of susceptible poultry to disease agents. Onsite litter and manure storage should limit attraction of wild birds, rodents, insects, and other animals.

(10) Replacement Poultry

Replacement poultry should be sourced from health-monitored flocks which are in compliance with NPIP guidelines. They should be transported in equipment and vehicles that are regularly cleaned, disinfected and inspected. Biosecurity protocols should be in place for equipment and personnel involved in the transport of replacement poultry.

(11) Water Supplies

It is recommended that drinking water or water used for evaporative cooling be sourced from a contained supply such as a well or municipal system. If drinking water comes from a surface

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water source, water treatment must be used to reduce the level of disease agents. If surfaces have been cleaned or flushed with surface water, subsequent disinfection should be employed to prevent disease transmission. If water treatment is not possible, a risk analysis should be performed to determine actions needed to mitigate risks.

(12) Feed and Replacement Litter

Feed, feed ingredients, bedding, and litter should be delivered, stored and maintained in a manner that limits exposure to and contamination by wild birds, rodents, insects, and other animals. Feed spills within the PBA (outside of the LOS) should be cleaned up and disposed in a timely fashion.

(13) Reporting of Elevated Morbidity and Mortality

Elevation in morbidity and/or mortality above expected levels, as defined by the biosecurity plan, should be reported as required in the site-specific biosecurity plan and appropriate actions should be taken to rule out reportable disease agents.

(14) Auditing

Auditing of the biosecurity principles is based on flock size as outlined in 9 CFR 53.10. Audits shall be conducted at least once every two years or a sufficient number of times during that period by the Official State Agency to ensure the participant is in compliance. Each audit shall require the biosecurity plan’s training materials, documentation of implementation of the NPIP Biosecurity Principles, corrective actions taken, and the Biosecurity Coordinator’s annual review to be audited for completeness and compliance with the NPIP Biosecurity Principles. An audit summary report containing satisfactory and unsatisfactory audits will be provided to the NPIP National Office by the OSAs.

Those participants who failed the initial document audit conducted by the NPIP OSA may elect to have a check audit performed by a team appointed by National NPIP Office including: an APHIS poultry subject matter expert, the OSA, and a licensed, accredited poultry veterinarian familiar with that type of operation. If these participants seek to be reinstated as being in compliance with the Biosecurity Principles by the NPIP OSA, they must demonstrate that corrective actions were taken following the audit by the team appointed by NPIP.